cd34 cord blood cells Search Results


adult cd34+ stem cells  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc adult cd34+ stem cells
Adult Cd34+ Stem Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adult cd34+ stem cells - by Bioz Stars, 2026-03
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dcs generated from umbilical cord blood cd34 + progenitor cells (haematopoietic stem cells)  (MatTek)
90
MatTek dcs generated from umbilical cord blood cd34 + progenitor cells (haematopoietic stem cells)
Dcs Generated From Umbilical Cord Blood Cd34 + Progenitor Cells (Haematopoietic Stem Cells), supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dcs generated from umbilical cord blood cd34 + progenitor cells (haematopoietic stem cells) - by Bioz Stars, 2026-03
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human cd34 + bone marrow cells  (Astarte Biologics)
90
Astarte Biologics human cd34 + bone marrow cells
Human Cd34 + Bone Marrow Cells, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd34 + bone marrow cells - by Bioz Stars, 2026-03
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cd34+ cells  (3H Biomedical)
90
3H Biomedical cd34+ cells
Cd34+ Cells, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34+ cells - by Bioz Stars, 2026-03
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cd34 + hspcs  (StemCyte Inc)
90
StemCyte Inc cd34 + hspcs
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Cd34 + Hspcs, supplied by StemCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34 + hspcs - by Bioz Stars, 2026-03
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umbilical cord blood cd34+ hematopoietic stem and progenitor-derived nk cells  (Snijders Scientific)
90
Snijders Scientific umbilical cord blood cd34+ hematopoietic stem and progenitor-derived nk cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Umbilical Cord Blood Cd34+ Hematopoietic Stem And Progenitor Derived Nk Cells, supplied by Snijders Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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umbilical cord blood cd34+ hematopoietic stem and progenitor-derived nk cells - by Bioz Stars, 2026-03
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human cord blood cd34+ cells  (SAS institute)
90
SAS institute human cord blood cd34+ cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Human Cord Blood Cd34+ Cells, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cord blood cd34+ cells - by Bioz Stars, 2026-03
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cord blood lin cd34 cells  (YUTAKA Engineering Corporation)
90
YUTAKA Engineering Corporation cord blood lin cd34 cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Cord Blood Lin Cd34 Cells, supplied by YUTAKA Engineering Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cord blood lin cd34 cells - by Bioz Stars, 2026-03
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cd34-positive cord blood cells  (Keio University Press Inc)
90
Keio University Press Inc cd34-positive cord blood cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Cd34 Positive Cord Blood Cells, supplied by Keio University Press Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cd34-positive cord blood cells - by Bioz Stars, 2026-03
90/100 stars
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cd34+ cord blood stem cell  (Hutzel Hospital)
90
Hutzel Hospital cd34+ cord blood stem cell
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Cd34+ Cord Blood Stem Cell, supplied by Hutzel Hospital, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34+ cord blood stem cell - by Bioz Stars, 2026-03
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cysteinyl leukotriene receptors by cd34+umbilical cord blood (cb) - progenitor cells  (Sepracor Inc)
90
Sepracor Inc cysteinyl leukotriene receptors by cd34+umbilical cord blood (cb) - progenitor cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Cysteinyl Leukotriene Receptors By Cd34+Umbilical Cord Blood (Cb) Progenitor Cells, supplied by Sepracor Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cysteinyl leukotriene receptors by cd34+umbilical cord blood (cb) - progenitor cells/product/Sepracor Inc
Average 90 stars, based on 1 article reviews
cysteinyl leukotriene receptors by cd34+umbilical cord blood (cb) - progenitor cells - by Bioz Stars, 2026-03
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umbilical cord blood cd34 cells  (Hayashibara Biochemical Laboratories)
90
Hayashibara Biochemical Laboratories umbilical cord blood cd34 cells
a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived <t>CD34</t> + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.
Umbilical Cord Blood Cd34 Cells, supplied by Hayashibara Biochemical Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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umbilical cord blood cd34 cells - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived CD34 + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.

Journal: Nature Cell Biology

Article Title: RBFOX2 recognizes N 6 -methyladenosine to suppress transcription and block myeloid leukaemia differentiation

doi: 10.1038/s41556-023-01213-w

Figure Lengend Snippet: a , Western blot assay to quantify RBFOX2 knockdown efficiency in NB4 cells. b-c , Flow cytometric analysis of CD11b + cell populations ( b ), and relative gene expression of CD14 and CD11b with qPCR quantification ( c ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) NB4 cells after treatment with 500 nM ATRA for 72 hr as compared to DMSO-treated cells. n = 3. d , Western blot assay to quantify RBFOX2 knockdown efficiency in MOLM13 cells. e-f , Flow cytometric analysis of CD11b + cell populations ( e ) and CD11b mRNA expression level ( f ) in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. n = 3. g , Wright-Giemsa staining of cytospin slides of control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) MOLM13 cells. Arrows indicate differentiated cells. Scale bar, 20 µm. h , Western blots showing the knockdown efficiency of RBFOX2 in human cord blood-derived CD34 + (CB-CD34 + ) cells. i , Flow cytometric analysis of the percentage of CD34 + cell population in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. Day 2 and Day 5 represent the number of days post-transduction. j , Quantification of the percentage of different hematopoietic lineage progenitor colonies in control (shNS) and RBFOX2 KD (sh RBFOX2 -1 and sh RBFOX2 -2) CB-CD34 + cells. n = 4. P values were calculated by two-way ANOVA, Dunnett’s multiple comparisons test. k-n , Flow cytometric analysis of megakaryocyte ( k ), erythroid ( l ), granulocyte ( m ) and monocyte ( n ) differentiation of CB-CD34 + cells (five days post-transduction) transfected with shNS or RBFOX2 shRNAs (sh RBFOX2 -1 and sh RBFOX2 -2). n , biologically independent samples. Data are presented as mean values +/− SEM. For c and f , two-sided P values by Student’s t-test.

Article Snippet: CD34 + HSPCs were extracted from UCB specimens, procured from StemCyte under the IRP protocol number 18147 approved by City of Hope.

Techniques: Western Blot, Knockdown, Gene Expression, Control, Expressing, Staining, Derivative Assay, Transduction, Transfection